|
Figure 2.
Fig. 2. Stereo view of the three-dimensional structure of the
Tkpro:SA-subtilisin complex. The entire structure of the
Tkpro:SA-subtilisin complex (PDB code: 2Z30) (a) and the
structure around Glu201 (b) are shown. Tkpro and SA-subtilisin
are colored pink and lime green, respectively. The Ca^2+ ions
are shown in cyan spheres. For the entire structure, two acidic
residues (Glu61 and Asp63), six C-terminal residues of Tkpro
(His64-Leu69), and Glu201 are indicated by stick models. Two
active-site residues (Asp115 and His153) and Ala324, which is
substituted for the active-site serine residue, are indicated by
yellow stick models. In these stick models, the oxygen and
nitrogen atoms are colored red and blue, respectively.
Glu61/Asp63-mediated hydrogen bonds are shown by orange broken
lines. N and C represent the N- and C-termini, respectively. For
the structure around Glu201, Glu201, Tyr31, and Phe33-Pro37 are
indicated by stick models, in which the oxygen and nitrogen
atoms are colored red and blue, respectively. Glu201-mediated
hydrogen bonds are shown by orange broken lines.
|
