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Figure 2.
Figure 2: JARID1A-PHD3, an essential motif for NJL-mediated
leukaemia, specifically recognizes H3K4me3/2 marks. a,
Capability of JARID1A-PHD3, PHF23-PHD and JARID1A-PHD1 (the
first PHD finger of JARID1A; Supplementary Fig. 1) to interact
with H3 peptides containing different states of Lys methylation,
in a peptide pull-down assay. JARID1A-PHD1 interacted with
H3K4me0 as BHC80-PHD^11. aa, amino acids. b, The crystal
structure of JARID1A-PHD3 (cyan) complexed with H3K4me3 peptide
(yellow), and a close-up view of the H3K4me3-binding channel
(inset) formed by two orthogonally aligned Trp residues. The
numeration of JARID1A-PHD3 and H3 residues is shown in red and
black, respectively. Protein Data Bank accession number, 3GL6.
c, Capability of wild-type (WT) or mutant JARID1A-PHD3 to bind
to H3K4me3/2. d, Co-immunoprecipitation showing that NJL
containing the wild-type, but not mutant (W1625A) PHD finger,
associated with H3K4me3 or H3 in transiently transfected 293
cells.
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