Figure 2.
(a) Real-time cellular uptake of pen-N3 visualized by
time-lapse microscopy. For a full movie, see Supplementary Movie
1. DIC, differential interference contrast; FITC, fluorescein
isothiocyanate. (b) Pen-N3 inhibits Wnt/TCF-dependent signaling.
Normalized TOPglow reporter activity was measured in
Wnt3a-stimulated HEK293S cells after 18 h of treatment with
pen-N3, compound FJ9 or pen as negative control. (c) Pen-N3
inhibits accumulation of -catenin
in HEK293S cells treated with Wnt3a. Cells were treated with
Wnt3a (100 ng ml^-1) for 18 h in the presence of the indicated
peptides (10 M),
lysed and processed for western blots. The antibody labels -catenin
(solid arrow) and also a nonspecific band (hollow arrow).
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Chem Biol
(2009,
5,
217-219)
copyright 2009.
-catenin
in HEK293S cells treated with Wnt3a. Cells were treated with
Wnt3a (100 ng ml^-1) for 18 h in the presence of the indicated
peptides (10 
M),
lysed and processed for western blots. The antibody labels