|
Figure 2.
ARNT PAS-B can exist in two distinct conformations. (A)
Widespread peak doubling is observed throughout ^15N-^1H HSQC
spectra of Y456T ARNT PAS-B, as seen by comparison with spectra
from the wild-type protein. Several doubled sites are
highlighted with blue circles in the Y456T spectrum. (B) The
E403 resonance from the Y456T ARNT mutant indicates two slowly
exchanging conformations, but the relative populations of these
can be perturbed by additional mutations. (C) Demonstration that
conformations 1 and 2 are in equilibrium is provided by Mono Q
separation of conformation 2 from a Y456T sample and monitoring
the reestablishment of the equilibrium with extended incubation.
|
