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Figure 2.
Fig. 2. The DFG motif and activation loop adopt different
conformations dependent on the bound ligand. (a) Superposition
of Nek2-T175A^ATPγS (yellow), Nek2^ADP (orange) and
Nek2-T175A^SU (green) protein structures shown as a ribbon in
two orientations related by a 90° rotation about the y-axis.
(b) Stereoview of Nek2-T175A^ATPγS (yellow carbon atoms) and
Nek2^ADP (orange carbon atoms) superposition at the DFG motif.
(c) Stereoview of Nek2-T175A^ATPγS (yellow carbon atoms) and
Nek2-T175A^SU (green carbon atoms) superposition at the DFG
motif. (d) Stereoview of Nek2-T175A^ATPγS (yellow carbon atoms)
and Nek2-T175A^Apo (light pink carbon atoms) superposition at
the DFG motif. (e) Schematic of the secondary structures adopted
by the four Nek2 structures and the amino acid sequence
surrounding the DFG motif in Nek2 and Aurora-A. (f)
Superposition of three Nek2 conformations of the DFG motif
together with the likely position adopted in the fully active
conformation based on the Aurora-A/TPX2 structure (magenta). The
orientation is that of panels (b) to (d) viewed from the bottom
left to the top right.
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