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Figure 2.
Figure 2. Detailed Structural Analysis of Nup85 and Seh1
(A) A ribbon representation of the Nup85 structure is shown in
rainbow colors along the polypeptide chain from the N to the C
terminus. The N-terminal domain invasion motif (DIM), the
α-helical solenoid domain, and their secondary structure
elements are indicated.
(B) The structure of the
Seh1•Nup85 heterodimer. The Nup85^DIM (magenta), the Nup85
α-helical solenoid domain (blue), the Nup85 αQ-αR connector
(red), the Seh1 β propeller (yellow), the disordered Seh1 5CD
loop (gray dots), and the Seh1 2CD loop (orange) are indicated;
a 90° rotated view is shown on the right. Dotted lines
represent disordered regions.
(C) Schematic representation
of the Seh1•Nup85 interaction. The Seh1 2CD loop, the Nup85
αQ-αR connector, and the DIM region are highlighted in orange,
red, and pink, respectively.
(D) The β propeller domain of
Seh1 in complex with the Nup85^DIM. Seh1 is shown in yellow, and
the six blades are indicated. The Nup85^DIM contributes one
strand to blade 6 and three strands to blade 7, completing the
β propeller.
(E) Schematic representation of the Seh1 β
propeller and its interaction with the Nup85^DIM.
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