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Figure 2.
(a) Sequence alignment of Tat from HIV-1 and EIAV. The basic
ARM that is required for RNA binding is highlighted in blue.
Cysteine and histidine residues proposed to form zinc-finger
motifs are indicated. (b) Tat interacts with the first cyclin
box repeat of CycT1. The basic ARM is suspended by its flanking
regions on helices H1 and H4 as well as H3 and H5 of CycT1, thus
wrapping around a CycT–specific insert in between H4 and H5
(yellow) that forms the Tat-interacting loop. (c) The C-terminal
leucine of Tat inserts into a hydrophobic groove formed between
helices H4 and H5 of CycT1. (d) Detailed view of the interaction
between Ile41, Asp42 and Tyr43 of Tat with Asn43, Asp47 of CycT1
and residues Arg251 and Lys253 of the CycT1 TRM.
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