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Figure 2.
Fig. 2. Nucleotide-bound state of Ran. (a) Identification of
nucleotide bound to Ran by reverse-phase HPLC confirmed the
presence of GDP. The stoichiometry of bound nucleotide was
assessed by titrating GDP and GTP standards (1.1, 0.58, 0.29,
0.14 nmol). This revealed that 0.71 nmol GDP bound to 0.86 nmol
Ran, which is consistent with 1:1 stoichiometry; GTP was not
detected. (b) Annealed omit F[o] − F[c] map near the
nucleotide bound to Ran (chain A) contoured at 5.0σ (left), and
anomalous map (right, contoured at 3.5σ) with a GDP molecule
and magnesium ion superimposed.
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