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Figure 2.
Positioning of 25-D1.16 and Hyb3 Fab fragments of TCR-like
antibodies over cognate pMHC complexes. The left panel shows the
25-D1.16-pOV8-K^b complex, and the right panel shows the
MAGE-A1-HLA-A1 complex. Fab heavy and light chains are colored
light and dark gray, respectively. The MHC heavy chain and
β[2]m are shown in cyan and dark blue, respectively. 25-D1.16
assumes an orientation that is common for TCRs, with the CDR1
and CDR2 loops contacting K^b helices and the CDR3 loops forming
direct contacts with the peptide. MAGE-A1-HLA-A1-bound Hyb3 has
an atypical orientation, tilting toward helix α[1] without
contacting helix α[2] and forming few direct contacts with the
peptide.
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