Figure 2.
Figure 2: Crystal structure of a Pol II–RdRP complex. a,
Ribbon model of Pol II (grey) with an initial, unbiased
difference Fourier electron-density map (cyan, contoured at 2.2
).
The map was calculated from protein model phases. It reveals the
RNA template–product duplex of scaffold RdRP-ss6
(Supplementary Fig. 2) in the active-centre cleft of Pol II. The
bridge helix is in green. The catalytic metal ion A is depicted
as a magenta sphere, and Zn^2+ ions as cyan spheres. The view is
related to that in Fig. 1a by a 90° rotation around a
vertical axis. b, Comparison of the RNA template–product
duplex in the RdRP EC with the DNA–RNA hybrid duplex in the
transcription EC^7. Protein structures were superimposed by
fitting the active-site aspartate loops.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nature
(2007,
450,
445-449)
copyright 2007.
).
The map was calculated from protein model phases. It reveals the
RNA template–product duplex of scaffold RdRP-ss6
(Supplementary Fig. 2) in the active-centre cleft of Pol II. The
bridge helix is in green. The catalytic metal ion A is depicted
as a magenta sphere, and Zn^2+ ions as cyan spheres. The view is
related to that in Fig. 1a by a 90° rotation around a
vertical axis. b, Comparison of the RNA template–product
duplex in the RdRP EC with the DNA–RNA hybrid duplex in the
transcription EC^7. Protein structures were superimposed by
fitting the active-site aspartate loops.