Figure 2.
Fig. 2. The 3'dG elongation process of Pol (A) The catalytic
residues, the metal ions and the dCTP are shown for the
pre-elongation complex. Phe^35 stacks upon the dNTPs
deoxyribose. The dNTP is unpaired. (B) Detailed view of the
lesion in the pre-elongation complex (cyan) superpositioned with
the lesion in the 3'dG elongation state (magenta). For clarity,
the finger domain has been omitted and the DNA molecules (cyan
and magenta) are viewed in a simplified form. Watson-Crick base
pairing revolves the DNA to position the 3'OH of the primer for
nucleophilic attack on the -phosphate of the
dNTP. (C) The DNA revolves from the pre-elongation state into
the first elongation state, forming a Watson-Crick base pair.
This aligns the 3'OH of the primer for nucleotidyl transfer. The
protein of the pre-elongation complex is omitted for clarity,
and the protein of the 3'dG elongation state is depicted in
gray. The DNA molecules are color coded as in Fig. 2B.
The above figure is reprinted
by permission from the AAAs:
Science
(2007,
318,
967-970)
copyright 2007.
(A) The catalytic
residues, the metal ions and the dCTP are shown for the
pre-elongation complex. Phe^35 stacks upon the dNTPs
deoxyribose. The dNTP is unpaired. (B) Detailed view of the
lesion in the pre-elongation complex (cyan) superpositioned with
the lesion in the 3'dG elongation state (magenta). For clarity,
the finger domain has been omitted and the DNA molecules (cyan
and magenta) are viewed in a simplified form. Watson-Crick base
pairing revolves the DNA to position the 3'OH of the primer for
nucleophilic attack on the 
-phosphate of the
dNTP. (C) The DNA revolves from the pre-elongation state into
the first elongation state, forming a Watson-Crick base pair.
This aligns the 3'OH of the primer for nucleotidyl transfer. The
protein of the pre-elongation complex is omitted for clarity,
and the protein of the 3'dG elongation state is depicted in
gray. The DNA molecules are color coded as in Fig. 2B.