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Figure 2.
Fig. 2. The binding site for I1–60His in bovine
F[1]-ATPase. (A and B) Ribbon and solid representations,
respectively, of the binding site groove formed from  -helices
and loops between them in the C-terminal domains of the  [DP]-,
[DP]-, and [TP]-
subunits, occupied by the long helix (residues 21–50) of
I1–60His (light blue). The N- and C-terminal ends of the
I1–60HIS are labeled N and C, respectively, in A. (B) The
orange side chains are those of the strictly conserved residues
Lys-24, Arg-25, and Glu-26 of I1–60His that do not interact
with F[1]-ATPase. (C and D) Interactions between residues 8–46
and F[1]-ATPase. (C) View from the side of the central stalk
showing the orientation of I1–60His relative to the  -subunit. The N- and
C-terminal ends of the I1–60HIS are labeled N and C,
respectively. (D) View down along the axis of the -subunit
showing the interaction of the short helix with the -subunit
and the interaction between the extended region formed by
residues 10–12 of I1–60His and side chains in the nucleotide
binding domain of the [E]-subunit.
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