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Figure 2.
Figure 2. Conformational Status of the Two Lobes of F1 TnC
(A) Structural comparison of the two lobes of F1 TnC with
the PDB picks up as the highest hits the apo N lobe of skeletal
TnC, which is in closed conformation (PDB code: 1skt; Tsuda et
al., 1999) and the holo C lobe of cardiac TnC in complex with
the corresponding N terminus of TnI (PDB code: 1ozs; Lindhout
and Sykes, 2003).
(B) Comparison of the HSQC spectra of apo
(blue) and holo (red) Lethocerus F1 TnC. The spectra were
recorded at 25°C on a 600 MHz spectrometer. The shift upon
Ca^2+ addition of the resonance of V144 is indicated.
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