Figure 2.
(a–c) Coomassie-stained gels of (a) glutathione
S-transferase (GST) fusions of hnRNP A1 NLS, hnRNP M NLS and M9M
bound to Kap 2
and then dissociated by 0.3–1.6 M
RanGTP; (b) GST–hnRNP A1 NLS bound to Kap 2
in the presence of buffer, maltose-binding protein (MBP)–hnRNP
A1 NLS, MBP–hnRNP M NLS or MBP-M9M; (c) interactions of
GST-Kap 1
with Kap ,
Kap in
the presence of importin- –binding
(IBB) domain of Kap ,
M9M or Kap
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Biol
(2007,
14,
452-454)
copyright 2007.
2
and then dissociated by 0.3–1.6 
M
RanGTP; (b) GST–hnRNP A1 NLS bound to Kap 
,
Kap