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Figure 2.
Figure 2. Fold and Structure of M48^USP-UbVME
(A) Ribbon
representation of the M48^USP structure (gray with the β
hairpin in orange) in complex with UbVME (green). The secondary
structure elements are labeled, and the side chains of catalytic
triad residues are shown in yellow.
(B) Electrostatic
surface potential representation of M48^USP with bound Ub shown
in a ribbon representation (top). Below, Ub, in an electrostatic
surface potential representation, was rotated 180° to show
the charge distribution on the face forming the interface. Note
the charge complementarity between the M48^USP acidic cleft and
the positively charged Ub C terminus.
(C) The final 2F[o]
− F[c] electron density map contoured at 1.3 σ indicates a
covalent bond between the catalytic C23 and the Cβ atom of the
former vinylmethylester moiety at the Ub C terminus.
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