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Figure 2.
Figure 2 Nuclear magnetic resonance titration shows the binding
interface between the polymerase  UBZ
domain and ubiquitin. (A) Chemical shift perturbations of the
UBZ domain plotted against residue number. Residues with
chemical shift changes greater than 2 
and 1 are
shown in orange (significantly perturbed) and yellow
(perturbed), respectively. (B) A surface representation of the
UBZ domain with significantly perturbed residues (labelled in
black) shown in orange and perturbed residues in yellow. (C)
90° right-handed rotation of (B). (D) Chemical shift
perturbations of residues in ubiquitin calculated and coloured
as in (A). (E) A surface representation of ubiquitin.
Significantly perturbed residues (shown in orange and labelled
in black) and perturbed residues (shown in yellow) are
distributed along the canonical hydrophobic surface defined by
residues V70, I44 (both labelled in red) and L8. (F) 90°
left-handed rotation of (E). Surface representations were
generated by PyMol (DeLano, 2002). pol, polymerase; UBZ,
ubiquitin-binding zinc finger.
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