|
Figure 2.
Figure 2. Structures of 3D^pol complexed with ribonucleotides
in the presence of Mg^2+. The NTPs make bridging interactions
between the fingers and palm domains. The bases are stacked on
Arg174 from the ring finger, the ribose interacts with Arg174
and Asp238 from the palm, and the triphosphate moiety interacts
with Arg163 and Lys167 from the ring finger and the backbone
amide of residue 236 from the palm. (a) A 2.35 Å
resolution 2F[o]–F[c] electron density map contoured at 1.5σ
around UTP. (b) A 2.25 Å resolution 2F[o]–F[c] electron
density map contoured at 1.5σ around CTP. (c) A 2.6 Å
resolution 2F[o]–F[c] electron density map contoured at 1.5σ
around ATP and (d) A 2.6 Å resolution F[o]–F[c] electron
density map (blue) contoured at 1.8σ around ATP. (e) A 2.35
Å resolution 2F[o]–F[c] electron density map contoured
at 1.5σ around GTP. (f) Comparison of the apo 3D^pol structure
(red) with all the 3D^pol–NTP structures (grey) showing the
minor shift in the ring finger position as result of
interactions with bound NTPs.
|
