Figure 2 - full size

 

Figure 2.
Fig. 2. Docking of the atomic model of MxiH into the EM density of the Shigella T3SS needle. (A) Molecule A of MxiH (ribbon) with the modeled N-terminal helix (cylinder) is shown as two views rotated by 90° about the long axis of the molecule. (B) End-on view of a 40-Å-thick section of the assembled needle. Each MxiH monomer is shown as in A and colored differently, starting from red and circling the needle to purple. EM density is shown as a blue mesh. (C) Stereo diagram of the side view of the assembled needle, colored as for B. Note that B and C are not shown at the same scale, and the needle assembly has an exterior diameter of 70 Å (16).