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Figure 2.
Figure 2: ING2 PHD finger recognizes H3K4me3. a, Six
superimposed ^1H,^15N heteronuclear single quantum coherence
(HSQC) spectra of PHD (0.2 mM) collected during titration of
H3K4me3 peptide are colour-coded according to the ligand
concentration (inset). b, The histogram displays normalized
^1H,^15N chemical shift changes observed in the corresponding
(a) spectra of the PHD finger. The normalized^27 chemical shift
change was calculated using the equation [(   H)^2
+ ( N/5)^2]^0.5,
where is
the chemical shift in parts per million (p.p.m.). Coloured bars
indicate significant change being greater than average plus
one-half standard deviation.
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