|
Figure 2.
FIGURE 2. Phosphopeptide complexes of Nck1-SH2 and
Nck2-SH2. A, Nck1-SH2 binds Tir12 (yellow), a 12-residue peptide
from the EPEC virulence factor Tir. Two N-terminal glutamate
residues of the peptide, not resolved in the electron density,
are excluded. The electrostatic surface reveals a deep,
positively charged (blue) phosphotyrosine binding pocket. B,
Nck2-SH2 complexed to Tir8 (green). C, schematic representation
of the Nck-SH2/Tir interaction. Chemical bonds of Tir12 are
shown in black, and those of residues of Nck are shown in
orange. Hydrogen bonds are indicated by dashed lines,
hydrophobic interactions by green arcs, and a cation-  interaction
by a blue dashed line.
|
