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Figure 2.
Figure 2. NMR structure of the Itk SH2 domain bound to the
Ac-ADpYEPP-NH[2] phosphopeptide. (a) The 20 lowest energy
structures of the Itk SH2/ADpYEPP complex. The peptide ligand is
shown in red and the SH2 domain in turquoise. Backbone heavy
atoms within the secondary structural elements over the entire
SH2 sequence were used for superpositions. (b) Ribbon diagram of
the energy minimized average structure of the Itk
SH2/phosphopeptide complex. (The view is identical with that
shown in (a).) Regular secondary structural elements and loop
regions are labeled. The location of Pro287 within the CD loop
is indicated. The phosphopeptide ligand (Ac-ADpYEPP-NH[2]) is
red and the phosphotyrosine (pY) and proline residue three
positions C-terminal (pY+3) are labeled. In both (a) and (b) the
first two residues of the phosphopeptide (AD) are not included
for clarity. No NOEs are observed between this region of the
peptide and the Itk SH2 domain.
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