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Figure 2.
Figure 2. Binding of transmission-blocking antibodies to Pvs25.
(a) 2A8 Fab bound via its heavy chain (dark blue) to the B
loop of Pvs25 domain 2. The light chain (light blue) does not
contact Pvs25. The B loop of Pvs25 domain 3 (red) extends up
from the plane of the triangle. (b) Monoclonal antibodies 1H10
and 1A5 do not bind in the presence of prebound 2A8. With Pvs25
immobilized on a Biacore chip, 2A8 was injected at (i) until
binding was saturated at (ii), and then the second antibody and
2A8 were injected at (iii) until (iv). Buffer without protein
was injected from (ii) to (iii) and from (iv) onward.
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