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Figure 2.
Figure 2. Three-dimensional structure of [Abu^19,Abu^34]-HsTx1
solved by ^1H-NMR. (A) Amino acid sequence of
[Abu^19,Abu^34]-HsTx1 and sequential assignments. X represents
the Abu residue that replaces the half-cystine residues.
Collected sequential NOEs are classified into strong, medium,
and weak NOE, and are indicated by thick, medium, and thin
lines, respectively. (B) Stereoviews of the 20 best molecular
structures superimposed for best fit. Only backbone atoms are
displayed (C[  ],
HN, CO) for clarity. (C) Molscript ribbon drawing of the average
minimized [Abu^19,Abu^34]-HsTx1 structure. The helix,
antiparallel  -sheet,
C[ ]backbone
trace and disulfide bridges are shown in red, blue, yellow, and
green, respectively. The six half-cystine residues are indicated
with their respective positions.
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