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Figure 2.
Figure 2. Amino acid sequence alignments of proposed
structurally corresponding residues of KOD DNA polymerases
(138-152, exonuclease domain; and 682-697, Thumb domain).
Acidic, basic and hydrophobic residues are shown in red, blue
and green, respectively. The boxed amino acid residues comprise
the loop structure in the exonuclease domain. The underlined
amino acid residues are the core sequence of the Exo I motif.
The abbreviations used are as follows: KOD, Thermococcus
kodakaraensis DNA polymerase; Tgo, Thermococcus gorgonarius DNA
polymerase; Tli, Thermococcus litoralis DNA polymerase;
9°N-7, Thermococcus sp. 9°N-7 DNA polymerase; Tag,
Thermococcus aggregans DNA polymerase; Pfu, Pyrococcus furiosus
DNA polymerase; RB69, bacteriophage RB69 DNA polymerase; T7,
bacteriophage T7 DNA polymerase.
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