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Figure 2.
Figure 2 Structure of the Exo84-RBD:RalA complex. (A) Ribbon
diagram of the Exo84-RBD:RalA complex. Exo84-RBD is colored in
red. RalA is colored in green, except that switch I (38 -50) and
switch II (69 -85) are highlighted in orange. The secondary
structures of RalA are numbered in a sequential order. The
GMPPNP is shown in a ball-and-stick representation and the Mg2+
is shown as a gray sphere. A close-up view of the boxed region
is shown in panel B, which is superimposed with a portion of
electron density map. (B) Representative portion of a  [A]-weighted
2F[o]-F[c] electron density map (contoured at 1.0 )
overlaid with the final refined model. The Exo84 and RalA
molecules are colored as in panel A and the selected residues
are shown in a ball-and-stick representation. (C) Ribbon
representation of the Exo84-RBD structure. The secondary
structure elements are numbered in a sequential order. (D)
Exo84-RBD:RalA complex forms a two-fold symmetry related dimer
in the crystal. The Exo84-RBD molecules are red and cyan, while
the RalA molecules are green and light purple, respectively.
Also shown are the two GMPPNP molecules. The putative
phospholipid-binding sites are indicated by green oval circles.
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