Figure 2.
Figure 2. Intrinsic Ca^2+-binding properties of the
synaptotagmin 4 and 11 C[2] domains. (a -f) 1H-15N HSQC
spectra obtained at different Ca^2+ concentrations for the rat
synaptotagmin 4 C[2]A (a) and C[2]B (b) domains, the D.
melanogaster synaptotagmin 4 C[2]A (c) and C[2]B (d) domains,
and the rat synaptotagmin 11 C[2]A (e) and C[2]B (f) domains.
The protein concentrations were 120 M
and the total Ca^2+ concentrations used (mM) were: a, 0, 0.2, 1,
5, 10, 20, 40, 80; b, 0, 20; c, 0, 5, 10, 20, 40, 80; d, 0, 0.2,
1, 3, 10, 20, 40; e, 0, 0.2, 1, 3, 5, 10, 20, 40, 80; f, 0, 20.
The 1H-15N HSQC spectra acquired in the absence of Ca^2+ and the
highest Ca^2+ concentrations are shown with multiple red
contours and multiple black contours, respectively, whereas
spectra obtained at intermediate Ca^2+ concentrations are shown
with single black contours.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nat Struct Mol Biol
(2004,
11,
844-849)
copyright 2004.
M
and the total Ca^2+ concentrations used (mM) were: a, 0, 0.2, 1,
5, 10, 20, 40, 80; b, 0, 20; c, 0, 5, 10, 20, 40, 80; d, 0, 0.2,
1, 3, 10, 20, 40; e, 0, 0.2, 1, 3, 5, 10, 20, 40, 80; f, 0, 20.
The 1H-15N HSQC spectra acquired in the absence of Ca^2+ and the
highest Ca^2+ concentrations are shown with multiple red
contours and multiple black contours, respectively, whereas
spectra obtained at intermediate Ca^2+ concentrations are shown
with single black contours.