|
Figure 2.
Figure 2 T7 DNA polymerase bypass of an 8oG lesion. Primer
extension reactions were performed with exo- (left) and
wild-type (right) T7 DNA polymerase with undamaged guanine (G)
and 8-oxoguanine (8oG) in comparison to controls containing no
enzyme. The images shown are for 3 min incubations of reaction
mixtures containing 200- to 400-fold excess of DNA over
polymerase. The most intense band in each lane is unreacted
primer, at least 80% of which remains unextended for all
efficiency reactions performed in this study. The location of
8oG within the template strand is as indicated and enhanced
images of products using 8oG are shown to the right of the boxed
images. The probability of insertion at each template site,
listed in percent to the right of each lane, is an average of 7
-16 determinations and is calculated as described previously
(Kokoska et al, 2003).
|
