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Figure 3.
Figure 3. Structure of ISWI-C.
(A) Two orthogonal views of ISWI-C. The HAND domain is
depicted in blue, SANT domain in green, SLIDE domain in yellow, and
the spacer helix connecting the SANT and SLIDE domains in
red. Disordered loops are depicted in gray. (A), (C), and Figures 4A
and 4C were produced with the programs Molscript (Kraulis, 1991) and
Raster3D (Merritt and Bacon, 1997).
(B) Sequence alignment of ISWI homologs from Drosophila melanogaster
(dmISWI), human (hSNF2H), yeast (scIsw1p), the Arabidopsis thaliana
homolog At5g18620 (atISWI), and the SANT domain of yeast
Ada2p. Conserved and conservatively substituted residues are
highlighted in blue. The secondary structure elements of the dmISWI
structure are indicated. SANT and SLIDE
domains as predicted by program SMART are underlined; brackets
correspond to the deletion mutants SANT and SLIDE. Dashed lines
indicate disordered regions.
(C) Electrostatic surface representation of ISWI-C. Depicted are the
surfaces pointing away from the nucleosome (I) and facing it (II) in
the hypothetical nucleosome/ISWI-C model described in the Discussion.
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