Figure 2.
Figure 2: Assignment strategy. Regions extracted from the
spectra of Fig. 1c and d (I -VI) superimposed on a PDSD spectrum
of uniformly labelled SH3 domain (black), the latter recorded
with a short mixing time of 15 ms (ref. 5. Part of the
assignment of the long-range correlations is reported in the
figure, and the lines define the different correlation patterns.
As an example, correlations between residues L33 and V44 are
shown. The correlations between the C and
C signals
of V44 and C and
C signals
of L33 are observed in panels I -III for 2-SH3, whereas
correlations between the methyl groups appear in the spectrum of
1,3-SH3 (panel IV). Of particular interest is the region around
50 p.p.m. (panel VI), where for 2-SH3 a large number of
cross-peaks due to the proline- signals
(P20 and P54) are observed, whereas in the corresponding area of
the U-SH3 sample (see Fig. 1b), no correlations are detected. In
the upper left corner, a schematic representation of an
antiparallel -sheet
is shown. The numbering of the residues (i and j) corresponds to
the numbering in Table 1.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
Nature
(2002,
420,
98-0)
copyright 2002.
and
C 
signals
of V44 and C 
signals
of L33 are observed in panels I -III for 2-SH3, whereas
correlations between the methyl groups appear in the spectrum of
1,3-SH3 (panel IV). Of particular interest is the region around
50 p.p.m. (panel VI), where for 2-SH3 a large number of
cross-peaks due to the proline- 
signals
(P20 and P54) are observed, whereas in the corresponding area of
the U-SH3 sample (see Fig. 1b), no correlations are detected. In
the upper left corner, a schematic representation of an
antiparallel