Figure 2.
Fig. 2. Oligomerization of -catenin
385-651 and its N- and C-terminal subdomains. Fragments at the
indicated concentrations were incubated with a 30-fold excess of
cross-linker. For the two amine-reactive cross-linking reagents
BS3 and DMS with spacer arms of 11.4 and 11.0 Å,
respectively, different cross-linking efficiency was observed.
Higher cross-linking efficiency results are shown here and were
obtained with DMS in the case of -catenin
507-632 and BS3 for -catenin
385-651 and -catenin
385-507. The first lane for the -catenin
385-651 fragment shows a sample in the absence of cross-linker.
Molecular mass markers are indicated on the left of each gel,
and apparent molecular mass of the fragments and cross-linking
products are shown on the right.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2002,
277,
18868-18874)
copyright 2002.
-catenin
385-651 and its N- and C-terminal subdomains. Fragments at the
indicated concentrations were incubated with a 30-fold excess of
cross-linker. For the two amine-reactive cross-linking reagents
BS3 and DMS with spacer arms of 11.4 and 11.0 Å,
respectively, different cross-linking efficiency was observed.
Higher cross-linking efficiency results are shown here and were
obtained with DMS in the case of