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Figure 1.
Figure 1. (a) A ribbon model of
the parent Zn finger showing Zn
2+
(blue sphere), ligands (two cysteine
and two histidine side-chains; light
blue), conserved leucine (L, light
blue), and central aromatic side-
chain (F10; red and asterisk). The C
terminus of the peptide is indi-
cated. (b) A CPK model of the
parent Zn finger showing the edge
of F10 in the potential crevice (dark
blue); the remainder of the surface
is shown in aquamarine. (c) A rep-
resentation of parent (F10) and
variant (L10) fingers. The side-
chains shown in (a) are shaded.
The numbering scheme refers
to the synthetic peptide (30
residues
19
). (d) CD spectra of Zn
2+
-
free peptides (F10, continuous line;
and L10 dashed line) and Zn
2+
-
bound peptides: wild-type (Phe;
filled circles) and variant (Leu;
open circles). CD spectra were
obtained at 25 ° C at a peptide con-
centration of 100 mM.
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