|
Figure 2.
Figure 2. Stereo view, topology diagram and superposition of
CheA and CheW. a, Stereo view showing the ensemble of
NMR-derived structures of CheW, with  -strands
in red and helices in blue. The 20 lowest energy structures
generated with CNS13 are superimposed to the mean. Disordered
regions of protein backbone were not used for the superposition.
b, Topology diagram of CheW showing the locations of secondary
structural elements within the primary sequence. -strands
are shown as red arrows; helices, as blue cylinders. c, Ribbon
diagram of CheW showing the superposition of the average
minimized NMR-derived structure of T. maritima CheW and CheA,
using homologous regions of secondary structure. The
superposition consists of CheW residues 10 -37, 51 -71, 79 -106,
124 -128 and 121 -141 from CheW (gold) and residues 538 -671
(543 -570, 581 -601, 610 -637, 652 -656 and 660 -670) from CheA
(blue). R.m.s. deviation of backbone atoms is 1.95 Å for
homologous regions. Residues corresponding to loops 1 and 2 and
the C-terminal helix of CheW are colored red. The stereo view
and ribbon diagrams were created with MOLMOL31, 32 and
RIBBONS33, respectively.
|
