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Figure 2.
Figure 2. The Activation Loop and Substrate Binding(a) The
structure of CDK2 (red) with a substrate peptide (purple) and a
nonhydrolizable analog of ATP (labeled ATP; only the terminal
phosphate group is visible; PDB code 1QMZ; [28]). Ser 5 is the
phosphorylatable serine in the substrate. Lysine 8 from the
substrate peptide interacts with phosphothreonine 160.(b)
Superposed structures of CDK2 (red), ERK2 (yellow; PDB code
2ERK; [27]) and GSK-3 (green; FS crystal form). The sulfate in
GSK-3 is labeled and superposes on phosphothreonine residues in
CDK2 and ERK2.(c) Coordinates of GSK-3 (green) are shown with a
substrate peptide (blue) modeled in the active site. The peptide
has been modeled on the peptide in the CDK2 crystal structure
(a) with the phosphothreonine residue at position 9 modeled with
its phosphate group occupying the position occupied by the
sulfate in the crystal structure of the FS crystal form of
GSK-3. The figure was drawn with GRASP [47].
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