Figure 2.
Fig. 2. Representative overlaid sensorgram for kinetic
study of -chymotrypsin
binding to BF9 measured by a BIAcore X system. BF9 was
immobilized on a CM5 sensor chip by amine coupling. The -chymotrypsin
was injected over the sensor chip at concentrations ranging from
10 to 500 nM: trace a, 10 nM; trace b, 30 nM; trace c, 50 nM;
trace d, 100 nM; trace e, 200 nM; trace f, 300 nM; trace g, 400
nM; trace h, 500 nM. Raw binding data were analyzed by
BIAevaluation Version 3.0 Software and fit to a 1:1 Langmuir
binding model. RU, response units.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2001,
276,
45079-45087)
copyright 2001.
-chymotrypsin
binding to BF9 measured by a BIAcore X system. BF9 was
immobilized on a CM5 sensor chip by amine coupling. The