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Figure 2.
Fig. 2. A, stereoview comparing interactions between EMBP
and sulfate ions S1 and S2 with those between EMBP and the
modeled disaccharide component of heparan sulfate (top).
Equivalent comparison involving S5 and S8 (bottom). Sulfate ions
from the EMBP structure are shown in black (oxygen atoms) and
orange (sulfur atom). The modeled carbohydrate is shown in
green. Hydrogen bonds between sulfate ions and EMBP are shown as
dashed lines; water molecules are colored cyan. B, nature and
topology of the carbohydrate-binding sites of EMBP and MBP
depicted as surface representations and colored by electrostatic
potential. Representation of EMBP surface in complex with
sulfate ions (left), with modeled disaccharide
IdoA(2-OSO[3])-GlcNSO[3](6-OSO[3]), a repeating component of
heparin and heparan sulfate (middle) and representation of rat
mannose-binding protein in complex with  -methyl-D-galactoside
(PDB Code: 1AFA) (right). The color coding is  15  T/e (red)
to +15 T/e (blue)
for EMBP, 10 T/e (red)
to +10 T/e (blue)
for MBP, where is the
Poisson-Boltzmann constant at temperature T (Kelvin) per
electron e, as generated by the program GRASP (61).
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