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Figure 2.
Figure 2. Binding of pYVNV to TEF1W Src SH2(a) Stereo
ribbon diagram of TEF1W Src SH2 complexed with the
phosphopeptide SpYVNVQN. A σ[A] weighted 2F[obs] − F[c]
electron density map at 1.0 σ is contoured around the
phosphopeptide.(b) In the same orientation as (a) but with the
SH2 domain represented as van der Waals spheres and the
phosphopeptide as a ball-and-stick model. The pYVNV peptide is
shown with bonds in orange, Src with atoms in white except
residue TrpEF1 (in magenta), TyrβD5 (in green), IleβE4 (in
peach), LysβD6 (in pink), and ArgαA2 and ArgβB5 (in cyan).(c)
Superposition of the TEF1W Src SH2 complexed with the SpYVNVQN
structure onto the native Grb2 SH2 complexed with KRFpYVNV. For
clarity, only the phosphopeptide residues resolved in both
structures are shown (SpYVNV for Src TEF1W, FpYVNV for Grb2).
Src TEF1W is in lighter shades (yellow, cyan, light green, and
magenta for Src, the peptide, TyrβD5, and TrpEF1, respectively)
while Grb2 is in darker shades (orange, blue, dark green, and
purple for Grb2, the peptide, PheβD6, and TrpEF1,
respectively). Coordinates for Grb2 SH2 were taken from the
crystal structure of Rahuel et al. ([25]) (RCSB ID code 1tze).
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