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Figure 2.
Figure 2. Water-mediated dimer interface and copper-mediated crystal contacts. (a) The ring of conserved waters
(red crosses) in a stereo view of a 5 Å slab of the final 2Fo
-
Fc electron density map (green basketweave, contoured
at 1.0 s). This view is rotated 90 ° around the vertical 2-fold axis from that shown in Figure 1(c), so that the two
Trp98 side-chains are across from each other rather than one in front of the other. (b) A schematic of the hydrogen-
bonding network in the dimer interface. In an example water-mediated interaction, the carbonyl oxygen atom of
Ala109 is hydrogen-bonded to water molecule 1244, which is itself further hydrogen bonded to the side-chain of
Asn120 in the second subunit of the same SOD dimer. This view, although schematic, is similar to that shown in
Figure 1(c). (c) Cu 300 lies on a special position and forms a crystal contact between symmetry-related 2-fold Sub1s
via His48 and His50. Electron density is contoured at 1 s (green) and 5 s (gold) above the mean.
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