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Figure 2.
Figure 2. Radiolabeled VEGF binding assay. [
125
I]VEGF was equilibrated (23 °C) with serial dilutions of unlabeled
VEGF and (a) Fab-12 or (c) Y0317. Fabs were captured with an anti-Fab antibody-coated immunosorbant plate.
Scatchard analysis (Munson & Rodbard, 1980) with a 1:1 binding model was used to calculate Kd of (b) 433 (±116)
pM for Fab-12 and (d) 19.8(±4.3) pM for Y0317.
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