|
Figure 1.
Fig. 1. Secondary structure diagram for rPrP. NOE
connectivities are denoted by lines, where the thickness
qualitatively represents the relative intensity (weak, medium,
or strong) of the NOE crosspeaks, and i designates the residue
number for rPrP. d[  N](i,
i+3) denotes an NOE between the  -proton of
residue i and the amide^ proton of residue i+3. The long-range
NOE line indicates by height the relative number of NOE
crosspeaks between residues i  i +  4. In the
consensus chemical shift index (59), contiguous up bars
designate -helix and
down bars designate  -strand.
Regions of secondary structure are depicted by helices for -helices
and^ broad arrows for -strands.
Hydrogen exchange was calculated from the intensity of proton
NOE crosspeak between the amide and water: open circles for
slow, filled for fast, and half-filled circles for medium
exchange rate. No circle indicates spectral overlap or proline.
The secondary structure diagram was created using the program
VINCE (60).
|
