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Figure 1.
Fig. 1. Pulse sequence of the 3D ^13C/^15N-edited
HMQC-NOESY-(HCACO)NH experiment. Narrow and wide bars represent
90° and 180° pulses, respectively. All pulses are applied
along the x-axis unless otherwise indicated. ^13C′ decoupling
was achieved using the SEDUCE-1 modulation [45] at an RF field
strength of 840 Hz. ^15N decoupling was accomplished using a
WALTZ-16 scheme [46]. The phase cycle is φ1=x, -x; φ2=2x,
2(-x); φ3=4x, 4(-x); φ4=8x, 8(-x); receiver=x, 2(-x), x, -x,
2x, 2(-x), 2x, -x, x, 2(-x), x. Quadrature detection in t[1]
and t[2] is achieved using the States-TPPI method [47] by
incrementing the phases of φ1 and φ4, independently. All other
parameters are similar to those described for the 3D ^15N-edited
NOESY-(HCACO)NH experiment [31].
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