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Figure 1.
Figure 1. Schematic Diagrams of the Site-Specific
Recombination Reaction(A) Site-specific recombination by
bacteriophage integrases requires two DNA substrates containing
related sequences at the site of strand exchange. For
bacteriophage HP1, the att P site is 418 bp long and, in
addition to two IHF binding sites, contains multiple integrase
binding sites: three type I sites and three type II sites that
exist as either direct or inverted repeating motifs ([18]). The
att B site is 18 bp long and contains an inverted repeat
sequence. The DNA chromosomes and their attachment sites are not
drawn to scale.(B) The steps of strand cleavage and exchange
proceed using a topoisomerase mechanism (see Introduction for
details). Adapted from Nash, 1996.
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