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Figure 1.
The multi-dentate interaction between harmonin and Sans. (A)
A schematic diagram showing the domain organizations of harmonin
and Sans. The NPDZ1 and SAM-PBM boundaries used in this study
are indicated. (B) GST-fusion protein-based pull-down assay
showing that NPDZ1 binds to the SAM domain alone and the SAM-PBM
of Sans. No interaction between the N-domain and the SAM domain
was detected. (C) Analytical gel-filtration analysis showing
that harmonin NPDZ1 and Sans SAM-PBM forms a 1∶1
stoichiometric complex. (D) ITC-based measurements of the
binding affinities of NPDZ1 with Sans PBM, SAM, and SAM-PBM.
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