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Figure 1.
Figure 1. Grb2SH3C Domain Overlay Blot of Gab2 Synthesized as
Peptide Array Detects Two SH3C-Binding Regions
A schematic
drawing of human Gab2 with its N-terminal pleckstrin homology
domain (PH) and the five R/KxxK motifs indicated is shown above
the peptide array panel. Gab2 was spot-synthesized as
overlapping 30 aa peptides, sliding 3 aa with each step.
Membrane was initially probed with 2.7 μg/ml GST to detect
nonspecific binding, then washed and incubated with anti-GST IgG
(rabbit), followed by HRP-coupled anti-rabbit IgG and ECL
detection. After visualization of nonspecific signals from the
detection system (indicated by dashed boxes), the filter was
reblocked and probed with 3.3 μg/ml GST-Grb2SH3C.
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