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Figure 1.
Structure of CaM bound to Ca[V]1.1 IQ (PDB code 2vay) is
represented in A–C. Structure of previously solved CaM bound
to Ca[V]1.2 IQ was obtained from the PDB with accession number
2f3y (d–F). Ribbon schematics represent CaM bound to Ca[V]1.1
IQ in A or to Ca[V]1.2 IQ in D. Peptides are represented as
green ribbons. Ca^2+ ions are represented as yellow spheres. The
amino terminus (N) of the peptide contacts the N-lobe (pink
ribbon) of CaM. C-lobe of CaM is blue. Linker ribbons that join
the lobes of CaM are light gray. Residues of Ca[V]1.1 IQ motif
that are different from Ca[V]1.2 IQ are represented as sticks on
peptide ribbon, except for the residues not observed in the
structure. B and E depict peptide stick residues contacting
molecular surface of the N-lobe. C and F show peptide residue
side chains (shown as sticks) in the vicinity of the C-lobe
hydrophobic pocket formed by methionines (shown as molecular
surface). B, C, E, and F, N-lobe carbons are pink, and carbon
atoms in the linker between N- and C-lobes of CaM are light
gray; C-lobe carbons are light blue (teal); peptide carbons are
green; oxygen atoms are red; sulfur atoms are orange; nitrogen
atoms are dark blue; waters are marine spheres; speculated
chloride is green sphere. The 2F[o] − F[c] maps representing
electron density are blue meshes. For best visibility, the 2vay
2F[o] − F[c] map is at 1.0 Å threshold and 2f3y map is
at presented 0.5 Å.
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