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Figure 1.
Three-dimensional organization of complex between kinesin
motor KCBP and regulatory Ca^2+-binding protein KIC. (A)
Schematic illustration of complex components. The fragments of
KCBP and KIC visualized in the crystal structure are traced in
orange for KCBP and in green for KIC. The domain structure of
KCBP is depicted. (B) Crystal structure of the complex of
kinesin KCBP and KIC solved at a resolution of 2.4 Å. The
motor (light orange) and KIC (green) are shown schematically.
ADP (light blue) and Ca^2+ ion (black) are space-filling models.
The regulatory domain of KCBP is highlighted in orange. (C and
D) Two views of the structure of KIC in the complex. In C, KIC
is shown in orientation allowing visualization of the part
corresponding to the central helix of the Ca^2+ sensors
(calmodulin, troponin C). The C-terminal part of the
“central” helix in KIC is less ordered than its N-terminal
half. For alignment of KIC with troponin C and calmodulin,
please see Table S2. In D, the EF-hands helices are indicated
(A, B, C, D) as the similar elements in the structures of Ca^2+
sensors. The β-sheet stabilizing the EF-hand pair is shown in
cyan.
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