|
Figure 1.
Structure of the PTHrP-antibody complex. a, view of the final
difference (F[obs] − F[calc]) electron density map (blue
transparent surface) in the vicinity of the hormone, at 2.0
Å resolution, calculated using protein phases derived from
the final model after omitting the hormone (and surrounding
atoms within 3 Å of the hormone) and performing a round of
simulated annealing to remove bias. The map is contoured at 3σ
with the final model overlaid upon it. b and c, view of the
complex with the hormone shown in red worm (main chain) and
ball-and-stick (side chain) fashion and the antibody drawn in
ribbon style (the heavy chain is in blue, and light chain is in
orange). b, polar interactions between the N-terminal end of the
hormone with antibody. c, polar and hydrophobic interactions
between the C-terminal end of the hormone with antibody. All
figures were drawn using MOLSCRIPT (42) and RASTER3D (43). The
electron density figure was displayed with the help of CONSCRIPT
(44).
|
