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Figure 1.
The 6D7A loop of the Nup214 NTD is essential for Ddx19
binding. (A) Domain organization of Nup214 and Ddx19. For Ddx19,
the N-terminal extension (NTE, orange) and the 2 RecA-like
domains (domain 1 and 2, green and light orange) are indicated.
For Nup214, the β-propeller domain (light blue) and its
C-terminal extension (CTE, yellow), the coiled-coil domain
(gray), and the C-terminal unstructured region containing
numerous FG-repeats (white) are indicated. The Nup214 NTD is
composed of the β-propeller domain followed by the CTE. The
bars above the domain structures mark the fragments of the
orthorhombic crystal form. (B) Gel filtration profiles of
full-length wild-type Ddx19 incubated with the Nup214 NTD,
Nup214 NTD 1–405, or Nup214 NTD Δ6D7A before injection.
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