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Figure 1.
Figure 1. DNA Binding and Distortion by Wild-Type HincII
(A) Two views of the structure of wild-type HincII (two subunits
shown as black or white ribbons) bound to cognate DNA (shown in
space filling with the recognition site DNA in light brown and
the center two base pairs of the YR step in cyan and magenta,
respectively, with flanking DNA in dark brown).
(B) Cartoon
depiction of B form DNA (left) with that of the center YR step
of the HincII DNA recognition sequence bound to wild-type HincII
(right), in which the DNA bases of the same strand are unstacked
and the purine bases from opposing strands exhibit greater
stacking surface area (dashed lines) forming the cross-strand
purine stack (CSPS).
(C) Numbering of the HincII
recognition sequence used throughout the text.
(D) Hydrogen
bonds between the side chain of N141 in wild-type HincII and
bound cognate DNA.
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