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Figure 1.
Figure 1. Schematic Representations of ALG-2 and Alix
Human ALG-2 has a Gly/Pro-rich N-terminal region followed by the
PEF domain containing five EF-hands (EF1–EF5) with eight α
helices (α1–α8). The first and second helices in each EF
hand are alternatively named, for instance, helix E1 and helix
F1, respectively. An alternatively spliced isoform (lacking
Gly121-Phe122) is designated ALG-2^ΔGF122 in this article.
Recombinant proteins of full-length ALG-2 and two types of
N-terminal deletion mutants (des3-20ALG-2 and des3-23ALG-2) were
crystallized. Alix has three distinct domains, named Bro1, V,
and Pro-rich. A 16-mer synthetic oligopeptide of the ABS in Alix
(Alix ABS peptide) was used for cocrystallization.
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