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Figure 1.
Fig. 1. The ING1 PHD finger recognizes H3K4me3. (a) Peptide
microarrays containing the indicated histone peptides were
probed with GST–ING1 PHD. Red spots indicate binding. me,
methylation; ac, acetylation; ph, phosphorylation; s, symmetric;
a, asymmetric. (b) Structure of the ING1 PHD finger in complex
with the histone H3K4me3 peptide. The PHD finger is shown as
solid surface. The histone peptide is depicted as ball-and-stick
model with C, O, and N atoms colored green, red, and blue,
respectively. (c) The PHD finger is shown as a ribbon with
residues mutated in human cancers colored brown. (d)
Interactions of the GST-fusion wild-type and mutant ING1 PHD
fingers with biotinylated histone peptides examined by Western
blot experiments.
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